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3 days post-transfection, cells were sorted by FACS to grow single clones, which were assessed on a build-up of neutral lipids by BODIPY-505 staining, followed by immunoblotting for expression of SQLE and sequencing of the SQLE gene locus to confirm Cas9-assisted partial gene deletion
Genomics 44 KumarS.TrivediP
Long-Term Safety Gaps Without human trials, its impossible to rule out risks like abnormal cell growth, cancer promotion, or autoimmune flare-ups
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It was demonstrated that cysteine S -conjugate -lyase highly expressed in the kidney was responsible for the observed nephrotoxicity (Zhang and Hanigan, 2003
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