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Ubiquitin, a conserved 76-amino acid protein, is first activated in an ATP-dependent manner by E1 ubiquitin-activating enzymes, then transferred to E2 conjugating enzymes, and finally attached to target proteins by E3 ubiquitin ligases, which provide the critical element of substrate specificity (Fig

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Pro-fibrotic markers yes-associated protein 1 (YAP1) and taffazin (TAZ) and autophagy marker p62 showed elevated expression in Gclc L-KO mice (Fig