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a 330 mcg dose = 0.099 mL = ~10 units
There were also too few options for a high-dose NAD+ supplement that also included enough TMG to offset the methyl donor shortage often seen in precursor supplementation
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The medium was changed to a serum-free medium (D6046, Sigma-Aldrich) 48 h after seeding, and sodium selenite (100 nM, S9133, Sigma-Aldrich) was added at the same time for maintaining the expression of GPX3
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