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Insulin receptor substrate proteins, chiefly IRS-1, are recruited and phosphorylated, providing binding sites for the regulatory subunit of phosphoinositide 3-kinase and leading through PIP3 and PDK1 to Akt, and onward to mTOR complex 1, GSK-3 and the FoxO transcription factors

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Analytical Validation and Quality Assurance Commercial MT2 preparations are supplied as lyophilized powder (e.g., 10 mg per vial) at high purity (typically 9899%), with quality confirmed by highperformance liquid chromatography (HPLC) and mass spectrometry (MS)

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