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Other considerations documented for tirzepatide in its FDA labeling include injection-site reactions, decreased appetite, and warnings such as the risk of thyroid C-cell tumors observed in rodents (a boxed warning) and pancreatitis and gallbladder events

[1] Cell Line:Cultured Sprague-Dawley rat Achilles tendon fibroblasts (passages 2-4) Concentration:2 g/mL Incubation Time:24 h Result:Increased F-actin (polymerized actin fibers, visualized as green fluorescence) levels notably relative to control cells with weak F-actin detection

This was associated with substantive mobility of the RAMP2 linker and TM helix, which had weaker interaction with the CTR TM bundle compared to RAMP1 and RAMP3 in AMY 1 R and AMY 3 R complexes

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