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doi: 10.1038/nature08021 148 McGowanPMSimedreaCRibotEJFosterPJPalmieriDSteegPSet al

The pentadecapeptide form sold for research was first characterized by Professor Predrag Sikiri's group at the University of Zagreb in the early 1990s, and the synthetic material supplied here is produced by solid-phase peptide synthesis to match that defined sequence

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Subsequently, a small-animal ultrasound system (Vevo F2, Canada) equipped with a linear 46-MHz probe was utilized to obtain long-axis M-mode aortic images and to assess aortic blood flow velocity using Doppler mode