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Weigh the tissue pieces and homogenize them in PBS at a ratio of 1:9 (e.g., 1g tissue to 9mL PBS) using a glass homogenizer on ice
Furthermore, PV VLPs have been successfully produced in recombinant expression systems, establishing a proof-of-principle for their use as potential vaccine candidates for a post-polio eradication era 15,16,17,18
See Peptide Half-Life Explained
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