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Hirose T, Shimizu K, Ogura H, Tasaki O, Hamasaki T, Yamano S, et al
Neuropsychopharmacology (2014) 39:223343
Proteins were then precipitated by the addition of the total sample volume to 4 volume liquid chromatographymass spectrometry (LCMS)-grade acetonitrile, as per the SP4 protocol 63 , followed by gentle shaking for 5 min and centrifugation at 3,220 g for 10 min