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It differs from the native 70 residue growth factor by an arginine-for-glutamate substitution at position 3 and a hydrophobic 13 residue N-terminal extension, MFPAMPLLSLFVN, which is itself a residual fragment of the methionyl porcine growth hormone fusion partner used to express the protein in Escherichia coli

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The areas adjacent to the patellar tendon (medial and lateral) and the soft tissue above and below the kneecap offer the best balance of injury proximity and subcutaneous depth

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