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The pancreas slides were stained with Hematoxylin and Eosin (H&E) to differentiate the architecture of pancreatic islets, and stained with anti-insulin antibody (1:5000 dilution) (Monoclonal Anti-Insulin, I2018-2mL, lot:084M4769, Sigma-Aldrich) for immunohistochemical staining of insulin in pancreatic tissue