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COX-2 is normally inactive, but can be turned on during inflammation to produce pro-inflammatory prostaglandins

Cells were then washed once with HBSS before application of alkaline TNE buffer (pH 8.6) containing (or not) 100 mM Mesna for 5 min at 4C to cleave residual surface GLP-1R

When tirzepatide activates both GIP and GLP-1 receptors, something remarkable happens: The GLP-1 component handles the usual suspects: reducing appetite, slowing down how quickly your stomach empties, and boosting insulin secretion when blood sugar rises