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The whole blood (30 mL) was layered on 20 mL Lymphoprep and centrifuged for 20 min at 400 g with the brake off, after which the upper plasma layer was removed and discarded

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Cardiovascular-Kidney-Metabolic Health: A Presidential Advisory From the American Heart Association

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Activating sirtuins through adequate NAD+ levels is associated with improved mitochondrial function, reduced inflammation, and extended cellular lifespan