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Isolated cells were characterized as fibroblasts by morphological appearance and expression pattern of specific proteins as described previously [24, 25]

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By immunohistochemical staining and western blot analysis, an increase in MMP-9 expression in the CS group was observed compared to that in the control group, and pretreatment with GHK-Cu reduced MMP-9 expression compared to that in the CS group (Figures 2AC)

Selenium is a cofactor for the enzyme glutathione peroxidase, which converts reduced glutathione to oxidized glutathione, which protects cells

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