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Participants Twelve women between the ages of 39 and 76 years with a mean age of 58.3 years participated in this trial at a private clinic

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Glucose/galactose adapted cells were cultured in DMEM containing 10% dFBS, 100 U ml 1 penicillin and 100 g ml 1 streptomycin, 1 mM sodium pyruvate and 25 mM glucose (Sigma-Aldrich, G7021-1KG) or 25 mM galactose (Sigma-Aldrich, G5388-500G) for a minimum of 1 month before being used experimentally

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These products are then re-esterified into phospholipids by lysophosphatidylcholine acyltransferase 3 (LPCAT3), thereby affecting the transmembrane properties of PUFAs and allowing their incorporation into cell membranes and lipids [34]