Li L, Ameri AH, Wang S, Jansson KH, Casey OM, Yang Q, et al
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Under normal physiological conditions, intracellular OS results from the abnormal production of ROS during typical mitochondrial respiration
One hundred microliters of the cell resuspension for each condition was transferred to a 96-well microtiter plate (Corning, 3904), from which the fluorescence (GFPexcitation = 475 nm and emission = 509 nm or RFPexcitation = 569 nm and emission = 600 nm) and absorbance (600 nm) were measured using a plate reader (BioTek Synergy H1)
Lee & Kim, 2022) Side Effects: dry mouth blurred vision & enlarged pupil causes presynaptic inhibition of norepinephrine release, which can affect autonomic control of the lens & pupil
Months 3-6: Peak Results This is where magic happens