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6a, TNF supplementation in the media for 8 h induced robust gene expression levels of both c-FLIP and c-IAP, which were significantly downregulated by a 2-h pretreatment with H 2 O 2 , BCNU, or diamide

Handle according to established laboratory safety procedures

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Based on the assumption that this is caused by a lack of oxygen in the inner ear, those affected are administered pure oxygen in a hyperbaric chamber

Crystallization of Pt GSTF8 in presence of ()-catechin and GSH Purified Pt GSTF8 in 30 mM Tris, pH 8, 200 mM NaCl, 1 mM EDTA was concentrated to 12.4 mg ml 1 using an Amicon Ultra-4 device (10 kDa cut-off) (Merck Millipore) and supplemented with 1 mM GSH and 25 mM ()-catechin immediately before crystallization