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Animal Model and Ethics Statement Wistar rats from the breeding colony at the Universidade do Extremo Sul Catarinense (UNESC), Brazil, were housed four times per cage under controlled conditions of temperature (22 1 C), relative humidity (4555%) and light-dark cycle (12:12 p.m., light on at 6 a.m.)

The inclusion bodies were solubilized in denaturing conditions and refolded by chemical methods resulting in active NPro that self-cleaves the oIGF-1 fusion partner

Cao B, Jin M, Brietzke E, McIntyre RS, Wang D, Rosenblat JD, Ragguett RM, Zhang C, Sun X, Rong C, et al

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