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You can give yourself the injection at any time of the day but there are some factors that you may want to think about

Levothyroxine absorption may also be reduced by magnesium supplements, requiring separation by at least 4 hours

For example, exosomal circUSP7 from NSCLC cells inhibits miR-934 in CD8 T cells, upregulates SHP2, suppresses effector cytokine secretion (e.g., TNF-, IFN-), and induces T-cell exhaustion, thereby impairing anti-PD-1 efficacy

JACC Basic Transl Sci 5:63244

While the exact time of day doesn't matter much, being consistent helps ensure you don't forget doses

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