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Protein in sample A and B was removed by precipitating with 200 L of cold ACN (containing 1 g/mL carbachol, the internal standard, IS) and centrifuging at 16,900 g for 20 min
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Ultrastructural analysis Graft samples were fixed in glutaraldehyde, dehydrated, impregnated, embedded in epoxy resin and acrylic, and cut into 60-nm sections using an ultramicrotome
For extended storage (beyond 6 months), refrigerate at 2-8C
Experimental Eye Research, 84(4), 663-669
Disclaimer: This article is for educational purposes only and does not constitute medical advice
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