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Similar to nucleated cells, the intracellular Ca 2+ concentration of erythrocytes is kept in the low nM range [74], whereas the extracellular Ca 2+ is orders of magnitude larger (low mM range)

Patients were to be randomized to 1200 mg, 450 mg, or placebo, administered intravenously daily for 3 days followed by weekly for 4 weeks

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Isolation and Storage of Peripheral Blood Mononuclear Cells, Plasma, and Red Blood Cells Peripheral Blood Mononuclear Cells (PBMCs), plasma, and red blood cells (RBCs) were isolated from whole blood by density gradient centrifugation using ficoll histopaque (Sigma, St

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