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The chip preparation procedure included: Conditioning: 50 mM NaOH Activation: EDC/NHS solution Capture: Strep-Tactin XT (50 g/mL in 10 mM sodium acetate, pH 4.5) Quenching: 1 M ethanolamine hydrochloride, pH 8.5 Wash: 0.1 M sodium borate, 1 M NaCl, pH 9.0 Twin-Strep-tagged ligands were captured on the chip using a 96-channel printhead under bidirectional flow for 750 s, followed by a 600 s baseline step in running buffer
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Short-term storage:28 C (standard refrigeration) Usage window:Use within 714 days for optimal peptide integrity
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