As one of the most abundant intracellular antioxidants, Glutathione participates in numerous biochemical processes associated with cellular protection, metabolic regulation, and oxidative stress response
Furthermore, Tim-AIII induces the accumulation of reactive oxygen species (ROS) and iron ions, as well as the production of malondialdehyde (MDA) and depletion of glutathione (GSH)
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To validate the effectiveness of A-RDN, protein levels of calcitonin gene-related peptide (CGRP), which is the dominant neurotransmitter for afferent renal nerves [37, 38], and tyrosine hydroxylase (TH), a marker for efferent sympathetic fibers in the renal pelvis were quantified