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2094-A REDUCING SCREEN FAILURE RATES FOR NASH CLINICAL TRIALS USING BALLOONING AND FIBROSIS ASSESSMENT WITH SECOND HARMONIC GENERATION/TWO-PHOTON EXCITATION MICROSCOPY AND ARTIFICIAL INTELLIGENCE ANALYSIS: RESULTS FROM THE TANDEM TRIAL Kutbuddin Akbary 1 , Ya-Yun Ren 1 , Dean Tai 2 , Naim Alkhouri 3 , Dominique Brees 4 and Jossy Kochuparampil 4 , (1)Histoindex Pte Ltd, (2)Histoindex Pte Ltd, Singapore, (3)Arizona Liver Health, Phoenix, AZ, (4)Novartis Pharma AG Background: Liver biopsy evaluation in non-alcoholic steatohepatitis clinical research network classification (NASH-CRN) by pathologists' interpretation are used in clinical trials for inclusion criteria and treatment endpoints
Included studies were classified as low-risk if their score was greater than 7, moderate-risk if their score was between 5 and 7, and high-risk if their score was less than 5
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Slices were permeabilized and blocked in TBS-T (0.3% Triton -100) with 5% donkey serum (Jackson ImmunoResearch Laboratories, West Grove, PA, #017-000-121) for 1 h at RT
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