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Every 30 min, the images were assessed for the number of tubules exhibiting more than 90% of SYTOX green positivity

Proper Storage Required Once reconstituted, store the vial in the refrigerator at 36F 46F (2 8C)

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Materials and methods Cell culture The cultivation of HCT116 cells was performed via McCoys 5A medium (Pricella, Wuhan, China), and SW480 cells were maintained in high-glucose DMEM medium (Solarbio, Beijing, China), supplemented with 10% serum (HyClone, USA) and 1% penicillin/streptomycin (Solarbio, Beijing, China)

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