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Cell lysates were harvested after 48 h of transfection and dual-luciferase reporter assays were performed using a Dual-Luciferase Reporter Assay System kit (Promega, Wisconsin, USA)

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Enhanced CYP3A4 activity in females results in faster clearance of CYP3A4 metabolized drugs such as midazolam, diazepam, nifedipine, verapamil, erythromycin, and methylprednisolone (Greenblatt et al

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