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Reactive astrocytes acquire heterogeneous characteristics that can contribute to brain disorders via the non-cell-autonomous mechanisms
After harvesting the cells by centrifugation at 4,000 g for 30 min, the cells were resuspended in lysis buffer (50 mM sodium phosphate, pH 8.0, 300 mM NaCl, 10 mM imidazole) and lysed using a sonication for 1 h with a 10-s pulse mode (10 s on and 10 s off) on ice
As we await more comprehensive data from ongoing research, the potential for this combined approach to offer a more effective and personalized treatment option is clear
The overall rate of treatment discontinuation due to adverse events was described as relatively low across published trials
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