Immunoprecipitation assay To check protein-protein interaction, HeLa cells were lysed using cell lysis buffer (20 mM TrisHCl pH 7.5, 1 mM EDTA, 150 mM NaCl, 1 mM EGTA, 1% Triton X100, 1 mM sodium orthovanadate, 2.5 mM sodium pyrophosphate, 1 mM PMSF and 1 protease inhibitor cocktail)
Cant have a conclusive opinion on this one
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This includes the expression of genes affecting IMF deposition-related signaling pathways and metabolic processes, as well as genetic variations that regulate the expression and functions of these genes, such as single nucleotide polymorphisms (SNPs), epigenetic modifications, and noncoding RNAs
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31 P nuclear magnetic resonance study of the brain in Alzheimer's disease J Neuropathol Exp Neurol 1988