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A Kofler bench is a metal strip with a temperature gradient (range from room temperature to 300 C)

As the upstream RLuc reporter is translated by cap-dependent scanning 58 , which is reported to be enhanced in YBX1 deficiency 59 , to compare the translational efficiency of the PRKAA1 5UTR in shCtrl and shGDH1 or shYBX1 cells, the FLuc activity of the empty reporter group was used for normalization, as previously described 38

NAD+ plays a crucial role in regulating immune cell function, DNA repair mechanisms, and inflammatory responses

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