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There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged Copper and peptide to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes
Think of it like jump-starting a car battery rather than keeping jumper cables permanently attached
Your provider may adjust based on your medical history
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Mucosal Immunol
This can increase your risk of heart disease and stroke
