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Basic Concentration Formula Volume (mL) = Mass of peptide (mg) / Target concentration (mg/mL) Example: - Vial contents: 5 mg AOD-9604 - Target stock concentration: 1 mg/mL - Required volume: 5 mg / 1 mg/mL = 5.0 mL Adjusting for Net Peptide Content If your COA reports a net peptide content below 100% (common when water and counterion weight is factored in), adjust for actual peptide mass: Actual peptide mass (mg) = Stated vial weight (mg) (Net peptide content % / 100) Example: - Vial: 5 mg stated weight - Net peptide content per COA: 85% - Actual peptide mass: 5 0.85 = 4.25 mg - Adjusted reconstitution volume for 1 mg/mL: 4.25 mL For precise in vitro dosing experiments, this correction step is important for reproducibility
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