It does not simply amplify it
After 3 h, the medium was removed and replaced with medium as follows: HUVEC and HDMEC were cultured in endothelial cell basal medium supplemented with 2% (HUVEC) or 1% (HDMEC) FCS, and 90 g ml 1 heparin, with or without hHGF or dimeric macrocycle
The whole process takes minutes
Elective peri-operative management of adults taking glucagon-like peptide-1 receptor agonists, glucose-dependent insulinotropic peptide agonists and sodium-glucose cotransporter-2 inhibitors: a multidisciplinary consensus statement: A consensus statement from the Association of Anaesthetists, Association of British Clinical Diabetologists, British Obesity and Metabolic Surgery Society, Centre for Perioperative Care, Joint British Diabetes Societies for Inpatient Care, Royal College of Anaesthetists, Society for Obesity and Bariatric Anaesthesia and UK Clinical Pharmacy Association
Freezing can degrade the formulation so do not freeze it
For women trying to conceive, even modest weight loss may help restore ovulation, particularly when insulin resistance plays a role