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Some people report a temporary sensation of pressure or flushing if lipotropic therapy is administered too quickly

It is unknown whether the drugs pharmacokinetic profile would allow for a once daily administration

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High abdominal pressure at 25, 30, 40, or 50 mmHg was maintained until sacrifice at 60 min (25 mmHg), 30 min (30 mmHg, 40 mmHg), or 15 min (50 mmHg)

For Oil Red O Staining, liver tissues were frozen in Tissue-Tek CRYO-OCT (Fisher Scientific) and cryostat tissue sections were cut at 7 m thicknesses, fixed with 10% formalin for 10 min, and stained following an Oil Red O procedure

The concentration of the nanobody was adjusted to 1 mg/mL, and then the alkoxy group on pAcF was connected to azide group in linker (50 molar excess) through oxime ligation