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MRP1/GS-X pump ATPase expression: is this the explanation for the cytoprotection of the heart against oxidative stress-induced redox imbalance in comparison to skeletal muscle cells

Cells were collected and washed with PBS once, and cellular fluorescence was determined by flow cytometry and data were collected by BD FACSCanto and analyzed with FlowJo V.10.1 software

dose coupling identifies an exposure relationship, not the molecular pathway

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Vial size directly determines reconstitution volume, dose precision, and protocol durationa 10mg vial reconstituted with 2mL bacteriostatic water yields 5mg/mL concentration, while the same 2mL added to a 15mg vial produces 7.5mg/mL

2 mM dl-propargylglycine (PPG, SigmaAldrich), 0.2 mM l-homocysteine (Hcy, SigmaAldrich), or 0.5 mM l-methionine (Met, Research Products International, Inc) was added as indicated