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This approach allowed for the continuation of OHCbl therapy or the adjustment of therapy required to improve metabolic control in our patients
This deferred maintenance could be the etiology of the defect in the glycosaminoglycan layer of the urothelium in patients with IC
Some products require refrigeration before first use, while others can be stored at room temperature for a limited time once in use
Then, prepared cell lysates were plated on a 96-well plate (Nunc) overnight at 4 C and incubated with 5% BSA PBST (NaCl 137 mM, KCl 2.7 mM, Na 2 HPO 4 10 mM, KH 2 PO 4 1.8 mM, 0.1% Tween 20) for 2 h at room temperature (RT)
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