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The cells were subsequently treated with DCFH-DA (10 M, Bio-lab) for 1 h in the dark, and washed three times with PBS

The treatment with the nanoreactors demonstrated to nearly fully eradicate an aggressive osteosarcoma inside the animal model

Flow cytometry analysis using DAF-FM DA showed that the NO level was 63.5% in the HO group, 53.7% in the g-CN group, and decreased to 12% in the Ru SA+AC group (Fig

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Free radicals also deplete glutathione levels, increasing free radical damage and creating oxidative stress