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doi: 10.1016/s0026-0495(99)90090-2 40 ZhangYSunBFengDHuHChuMQuQet al
Despite their significant clinical potential, they are associated with certain limitations, including suboptimal pharmacokinetics and patient adherence challenges, necessitating the development of advanced drug delivery systems to optimize efficacy and improve compliance
For experiments in 384-well plates, wells were aspirated, rinsed with PBS and incubated with a fixative and staining solution containing 3.7% formaldehyde (Fisher Scientific, F75P-1GAL) and 5 g ml 1 Hoechst 33342, trihydrochloride, trihydrate (Invitrogen, H1399)