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A prolonged exposure to pharmacological agents can rapidly compromise neutrophils viability and activation statewe chose this standardized incubation to avoid introducing variability across conditions, and we selected inhibitor concentrations within the reported range of EV formation impairment (summarized in Table 1)

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Meanwhile, other classes such as insulins and dipeptidyl peptidase-4 (DPP4) inhibitors are rarely used as first-line therapies today, instead serving as follow-on options when additional control is needed