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Luminescence-based activity assays suggest that this cleavage site shift evolved during higher-stringency rounds of selection on procaspase-1

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At 24 and 36 hpi, the cells were fixed, permeabilised, immunolabelled with His-FITC, Flag-Alexa Fluor 647, and Myc-rhodamine, and processed for immunofluorescence microscopy

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Anesthesiology 2017;126(3):376393