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Metformin has also been shown to improve insulin secretion from rat islets chronically exposed to high levels of NEFA or glucose [21], suggesting a direct effect on pancreatic beta cells independent of its glucose-lowering action

As predicted by trRosetta, the fusion of HSA-binding DARPin to GLP-1 using a rigid helical linker does not alter the tertiary structure of the fusion partner, and each moiety of the fusion proteins will most likely retain their conformational properties and relevant biological functions as the native protein