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Mice were euthanized and tissues collected at 6 h (two experimental cohorts, saline n = 4, diquat n = 4) and 24 h (four experimental cohorts, saline n = 8, diquat n = 14) following treatment

Different clinical symptoms reported in individual patients with 5-oxoprolinase deficiency are renal stone formation, enterocolitis, mental retardation, neonatal hypoglycemia, microcytic anemia and microcephaly [4045]

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This feature was exploited for selective and sensitive quantification of creatinine (CRN) which is an important diagnostic parameter