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Methotrexate is metabolized and stored in hepatocytes in its polyglutamated form, leading to a longer presence of MTX within the cells, which has been suggested as a possible mechanism for MTX-induced liver damage (Bath et al
For fixation, an equal volume of PBS (Thermo Fisher Scientific) + 8% paraformaldehyde (Electron Microscopy Sciences) was added to the medium for 15 min, after which the samples were washed three times with PBS
Carbocyanine-3-chlorophenylhydrazone (CCCP) activated the PINK/Parkin pathway and reversed the regulation of mitophagy and mitochondrial function by SL
Discuss the technical differences between point of care testing and laboratory testing and the influence on patient care
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