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The blood samples were diluted 1:2 in phosphate-buffered saline (PBS), layered onto a separation solution (Biocoll 1.077 g/ml, Biochrom AG, Berlin, Germany), and centrifuged (800 x g, 20 min, 20C, Multifuge X3 F2, Thermo Fisher Scientific, Dreieich, Germany)
L., et al., -lipoic acid reduces fatty acid esterification and lipogenesis in adipocytes from overweight/obese subjects
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