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The concentrated product was loaded onto a Superdex 200 increase 10/300 GL column with SEC running buffer containing 20 mM HEPES, pH 7.5, 100 mM NaCl, 10 mM MgCl 2 , 100 M TCEP, 2 M peptide 20, 0.00075% LMNG, 0.00025% GDN and 0.0002% (w/v) CHS

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mean weight was 90 kg