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The standard vehicle in peptide research is bacteriostatic water sterile water containing 0.9% benzyl alcohol, which inhibits bacterial growth and allows multi-use vials to remain stable for extended research periods

The cell proliferation viability, ROS production, cell apoptosis, the expression levels of proteins, and the intracellular localization were detected by abovementioned methods

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After 2 h survival, mice were terminally anaesthetised with 100 mg/kg pentobarbital and transcardially perfused with 30 ml heparinised 0.1 M PBS followed by 4% PFA in 0.1 M PBS

Its a silent variable that can derail an entire project

Hojberg PV, Vilsboll T, Rabol R, Knop FK, Bache M, Krarup T, Holst JJ, Madsbad S