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Analytes were dissolved in 100% DMSO at a concentration of 50 mM, and subsequently diluted in sterile HEPES 20 mM, pH 7.4, NaCl 150 mM, 0.005% surfactant Tween20 to yield 2% DMSO final concentration (HSP-2%D buffer) and final analyte concentration: 1 mM

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