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Sample preparation (B) In an alternate protocol, the frozen liver tissue was directly lysed by probe sonication (2 10 pulse, 40% power output) in 4 M urea/DPBS and briefly cleared of debris by centrifugation (5000 g , 5 min, 4 C)

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The test subjects were divided into two groups, where one group was presented with placebo for 84 days and the rest were presented daily with various concentrations of the peptide, for up to 84 days

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