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Acquisition of 1 H-NMR spectra and metabolic profiling The lyophilized metabolic extracts were suspended in 0.6 ml of deuterated water (Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA) containing phosphate buffer (100 mM) and TSP (3-3-(trimethylsilyl)-2,2,3,3-tetradeuteropropionic acid or TMSP- d 4 , Cambridge Isotope Laboratories) (0.5 mM) and inserted into a 5 mm NMR tube for immediate acquisition

During the development of osteoarthritis, the synovium undergoes interstitial vascularization, fibrosis and hyperplasia (167), and macrophages and fibroblasts in the synovium can release proinflammatory cytokines, etc., which cause synovitis and cartilage degeneration

Cross-pathway research that combines GHRH-receptor activation with ghrelin-receptor activation typically pairs a GHRH analog like Sermorelin with a GHS such as Ipamorelin

284 , F840F851 (2003)

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