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The column was pre-equilibrated with 10 CV of DPBS (Sigma-Aldrich)/0.05% (v/v) sodium azide (Sigma-Aldrich), before the supernatant adjusted to pH 7.2 was loaded onto the column
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Ans : Typically between 4 to 12 weeks, depending on your body, dosage, and form used
Article Details How to Cite AZIZI-CHEKOSARI ., BOUYE ., & SEIDAVI, A
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