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In the second step adding NADPH and glutathione reductase results in conversion of all GSSG to GSH which reacts subsequently with DTNB and fluorescent or colorimetric substrate to be quantified
Keap1 responds to oxidative stress signals by freeing Nrf2
PMID 11934214
Bununla birlikte madde, WADA yasak listesinde yer almakta ve yksek performansl sporda yasaklanmaktadr [6](#ref-6)
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[5, 6] It is considered the main redox buffer in human cells owing to its large amount of reducing equivalents, [7] and is an important enzyme cofactor that serves as a neuromodulator in the central nervous system
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