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Single-stain controls were used to ensure the absence of fluorescence channel bleed-through and to confirm that spectral compensation was not required
It is required for the function of chaperones such as protein disulfide isomerases and calreticulin, which facilitate disulfide bond formation and ensure proper protein conformation 59
The current standard operating procedure by the European Center for the Validation of Alternative Methods (ECVAM) recommends the use of MTT reduction as parameter to make prediction on the skin irritancy of substances using EpiSkin TM test method, while IL-1 endpoint is regarded as a useful adjunct to MTT assay 48
It can reduce cholesterol and prevent and treat arteriosclerosis
Some research has shown supplemental doses of 500 to 1,000 mg per day may boost glutathione levels
No deproteination step is required, and results are achieved in as quickly as 30 minutes
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